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From 2010.igem.org

CLSM IMAGING

- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution.
- Spread the samples on the slide with the help of an inoculation loop.
- Wait for the sample to dry.
- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide.
- Observe the slides under Brightfield 100X

- Settings for GFP:

                      
 HFT 488 
                      
 Ch 1
                      
 LP 505 
                      
 Line 8
                      
 543 nm excitation
                      
 Laser power 5%

- Settings for RFP:

                      
 HFT 488/543 
                      
 Ch 1
                      
 LP 560 
                      
 Line 8
                      
 543 nm excitation
                      
 Laser power 10%